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molibresib 10676  (Cayman Chemical)

 
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    Cayman Chemical molibresib 10676
    Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 <t>[molibresib</t> (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).
    Molibresib 10676, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molibresib 10676/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    molibresib 10676 - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation"

    Article Title: Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation

    Journal: Cells

    doi: 10.3390/cells11182839

    Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 [molibresib (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).
    Figure Legend Snippet: Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 [molibresib (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).

    Techniques Used: Inhibition, Imaging, Fluorescence, Microscopy, Labeling, Stable Transfection, Expressing, Staining, Confocal Microscopy, Transfection, Western Blot

    Loss of ATG7 blocks molibresib-induced pexophagy in HeLa cells. ( A , B ) HeLa cells pre-treated with molibresib (10 µM) for 48 h were further incubated with or without bafilomycin A1 (Baf. A1, 25 nM) for 6 h. Then, the cells were harvested to analyze by Western blotting with indicated antibodies ( A ); the cells were stained with anti-ABCD antibody (red) and Hoechst 33,342 (blue). The number of peroxisomes per cell was calculated by assessing > 100 cells ( B ); ( C ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h. The cells were stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; ( D ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h, and then harvested for analysis by Western blotting with the indicated antibodies; ( E ) HeLa cells were transiently transfected with scrambled siRNA (Sc) or validated siRNA targeting for NBR1 (siNBR1) or p62 (sip62). After 24 h, the cells were further treated with molibresib (10 µM) for 48 h and stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; the scale bar indicates 20 µm. (Data indicate means ± S.E.M. * p < 0.05, *** p < 0.001, ns: not significant).
    Figure Legend Snippet: Loss of ATG7 blocks molibresib-induced pexophagy in HeLa cells. ( A , B ) HeLa cells pre-treated with molibresib (10 µM) for 48 h were further incubated with or without bafilomycin A1 (Baf. A1, 25 nM) for 6 h. Then, the cells were harvested to analyze by Western blotting with indicated antibodies ( A ); the cells were stained with anti-ABCD antibody (red) and Hoechst 33,342 (blue). The number of peroxisomes per cell was calculated by assessing > 100 cells ( B ); ( C ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h. The cells were stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; ( D ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h, and then harvested for analysis by Western blotting with the indicated antibodies; ( E ) HeLa cells were transiently transfected with scrambled siRNA (Sc) or validated siRNA targeting for NBR1 (siNBR1) or p62 (sip62). After 24 h, the cells were further treated with molibresib (10 µM) for 48 h and stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; the scale bar indicates 20 µm. (Data indicate means ± S.E.M. * p < 0.05, *** p < 0.001, ns: not significant).

    Techniques Used: Incubation, Western Blot, Staining, Knock-Out, Transfection

    Inhibition of BRD4 promotes pexophagy by increasing the ROS levels in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were incubated with fluorescent DCFH-DA dye (10 µM, 10 min). The cells were imaged (scale bar 50 µm), and DCFH-DA fluorescence intensity was measured using image processing software ImageJ; ( B ) HeLa/pexo-HyPer cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The level of peroxisomal H 2 O 2 was imaged (scale bar, 50 μm) and measured using the fluorescence intensity of HyPer-PTS1; ( C ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the number of peroxisomes in cells. Representative cell images are presented (C, scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. ** p < 0.01, *** p < 0.001, ns: not significant).
    Figure Legend Snippet: Inhibition of BRD4 promotes pexophagy by increasing the ROS levels in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were incubated with fluorescent DCFH-DA dye (10 µM, 10 min). The cells were imaged (scale bar 50 µm), and DCFH-DA fluorescence intensity was measured using image processing software ImageJ; ( B ) HeLa/pexo-HyPer cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The level of peroxisomal H 2 O 2 was imaged (scale bar, 50 μm) and measured using the fluorescence intensity of HyPer-PTS1; ( C ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the number of peroxisomes in cells. Representative cell images are presented (C, scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. ** p < 0.01, *** p < 0.001, ns: not significant).

    Techniques Used: Inhibition, Incubation, Fluorescence, Software

    Inhibition of BRD4 activates ATM to promote pexophagy in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The cells were harvested and analyzed by Western blotting with the indicated antibodies; ( B ) HeLa cells were treated with molibresib (10 µM) with or without KU55933 (10 µM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the peroxisomes. Representative cell images are presented (scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. *** p < 0.001, ns: not significant).
    Figure Legend Snippet: Inhibition of BRD4 activates ATM to promote pexophagy in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The cells were harvested and analyzed by Western blotting with the indicated antibodies; ( B ) HeLa cells were treated with molibresib (10 µM) with or without KU55933 (10 µM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the peroxisomes. Representative cell images are presented (scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. *** p < 0.001, ns: not significant).

    Techniques Used: Inhibition, Western Blot



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    molibresib 10676  (Cayman Chemical)
    90
    Cayman Chemical molibresib 10676
    Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 <t>[molibresib</t> (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).
    Molibresib 10676, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molibresib 10676/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    molibresib 10676 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 [molibresib (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).

    Journal: Cells

    Article Title: Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation

    doi: 10.3390/cells11182839

    Figure Lengend Snippet: Inhibition of BRD4 promotes pexophagy in RPE cells. ( A ) RPE/mRFP-EGFP-SKL cells were treated with inhibitors of BRD4 [molibresib (10 µM), I-BET151 (10 µM), dBET1 (10 µM)] for 72 h. The cells were fixed for imaging under a fluorescence microscope. The numbers of cell with EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(-) and mRFP(+)-labeled autolysosomes, which displayed the peroxisomal reporter mRFP-EGFP-SKL due to lysosomal delivery, were counted and are presented as percentages; ( B ) HeLa cells stably expressing pmTurquiose2-Peroxi, pmTurquiose2-Mito, pmTurquiose2-ER, or pmTurquiose2-Golgi were treated with molibresib (10 µM) for 72 h and stained with DRAQ (red). Cellular organelles were imaged by confocal microscopy. The scale bar indicates 20 µm; ( C , D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with siRNA targeting BRD4 (siBRD4), and then, EGFP and mRFP fluorescence was imaged and quantified ( C ); reduced expression of BRD4 by siRNA was verified by Western blotting ( D ). (Data indicate means ± S.E.M. *** p < 0.001).

    Article Snippet: Molibresib (10676), I-BET151 (11181) and dBET1 (18044) were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA).

    Techniques: Inhibition, Imaging, Fluorescence, Microscopy, Labeling, Stable Transfection, Expressing, Staining, Confocal Microscopy, Transfection, Western Blot

    Loss of ATG7 blocks molibresib-induced pexophagy in HeLa cells. ( A , B ) HeLa cells pre-treated with molibresib (10 µM) for 48 h were further incubated with or without bafilomycin A1 (Baf. A1, 25 nM) for 6 h. Then, the cells were harvested to analyze by Western blotting with indicated antibodies ( A ); the cells were stained with anti-ABCD antibody (red) and Hoechst 33,342 (blue). The number of peroxisomes per cell was calculated by assessing > 100 cells ( B ); ( C ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h. The cells were stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; ( D ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h, and then harvested for analysis by Western blotting with the indicated antibodies; ( E ) HeLa cells were transiently transfected with scrambled siRNA (Sc) or validated siRNA targeting for NBR1 (siNBR1) or p62 (sip62). After 24 h, the cells were further treated with molibresib (10 µM) for 48 h and stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; the scale bar indicates 20 µm. (Data indicate means ± S.E.M. * p < 0.05, *** p < 0.001, ns: not significant).

    Journal: Cells

    Article Title: Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation

    doi: 10.3390/cells11182839

    Figure Lengend Snippet: Loss of ATG7 blocks molibresib-induced pexophagy in HeLa cells. ( A , B ) HeLa cells pre-treated with molibresib (10 µM) for 48 h were further incubated with or without bafilomycin A1 (Baf. A1, 25 nM) for 6 h. Then, the cells were harvested to analyze by Western blotting with indicated antibodies ( A ); the cells were stained with anti-ABCD antibody (red) and Hoechst 33,342 (blue). The number of peroxisomes per cell was calculated by assessing > 100 cells ( B ); ( C ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h. The cells were stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; ( D ) wild-type and ATG7-knockout HeLa cells were treated with molibresib (10 µM) for 48 h, and then harvested for analysis by Western blotting with the indicated antibodies; ( E ) HeLa cells were transiently transfected with scrambled siRNA (Sc) or validated siRNA targeting for NBR1 (siNBR1) or p62 (sip62). After 24 h, the cells were further treated with molibresib (10 µM) for 48 h and stained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue). The number of peroxisomes per cell was calculated by assessing approximately 100 cells; the scale bar indicates 20 µm. (Data indicate means ± S.E.M. * p < 0.05, *** p < 0.001, ns: not significant).

    Article Snippet: Molibresib (10676), I-BET151 (11181) and dBET1 (18044) were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA).

    Techniques: Incubation, Western Blot, Staining, Knock-Out, Transfection

    Inhibition of BRD4 promotes pexophagy by increasing the ROS levels in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were incubated with fluorescent DCFH-DA dye (10 µM, 10 min). The cells were imaged (scale bar 50 µm), and DCFH-DA fluorescence intensity was measured using image processing software ImageJ; ( B ) HeLa/pexo-HyPer cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The level of peroxisomal H 2 O 2 was imaged (scale bar, 50 μm) and measured using the fluorescence intensity of HyPer-PTS1; ( C ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the number of peroxisomes in cells. Representative cell images are presented (C, scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. ** p < 0.01, *** p < 0.001, ns: not significant).

    Journal: Cells

    Article Title: Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation

    doi: 10.3390/cells11182839

    Figure Lengend Snippet: Inhibition of BRD4 promotes pexophagy by increasing the ROS levels in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were incubated with fluorescent DCFH-DA dye (10 µM, 10 min). The cells were imaged (scale bar 50 µm), and DCFH-DA fluorescence intensity was measured using image processing software ImageJ; ( B ) HeLa/pexo-HyPer cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The level of peroxisomal H 2 O 2 was imaged (scale bar, 50 μm) and measured using the fluorescence intensity of HyPer-PTS1; ( C ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the number of peroxisomes in cells. Representative cell images are presented (C, scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. ** p < 0.01, *** p < 0.001, ns: not significant).

    Article Snippet: Molibresib (10676), I-BET151 (11181) and dBET1 (18044) were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA).

    Techniques: Inhibition, Incubation, Fluorescence, Software

    Inhibition of BRD4 activates ATM to promote pexophagy in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The cells were harvested and analyzed by Western blotting with the indicated antibodies; ( B ) HeLa cells were treated with molibresib (10 µM) with or without KU55933 (10 µM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the peroxisomes. Representative cell images are presented (scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. *** p < 0.001, ns: not significant).

    Journal: Cells

    Article Title: Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation

    doi: 10.3390/cells11182839

    Figure Lengend Snippet: Inhibition of BRD4 activates ATM to promote pexophagy in HeLa cells. ( A ) HeLa cells were treated with molibresib (10 µM) with or without NAC (1 mM) for 48 h. The cells were harvested and analyzed by Western blotting with the indicated antibodies; ( B ) HeLa cells were treated with molibresib (10 µM) with or without KU55933 (10 µM) for 48 h. Then, the cells were immunostained with anti-ABCD3 antibody (red) and Hoechst 33,342 dye (blue) to count the peroxisomes. Representative cell images are presented (scale bar 20 µm). The experiments were repeated at least three times. (Data indicate means ± S.E.M. *** p < 0.001, ns: not significant).

    Article Snippet: Molibresib (10676), I-BET151 (11181) and dBET1 (18044) were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA).

    Techniques: Inhibition, Western Blot